Gabriel Rosenblum

Weizmann Institute
Postdoc

Research

Systems and Approaches
Biochemistry and Biophysics

Interests
Protein structure and dynamics

Summary
My thesis work focused on understanding the mechanistic properties of the zinc endopeptidase, Matrix Metalloproteinase-9 (MMP-9), involved in many pathological processes such as cancer and autoimmune diseases. To address structural and dynamical properties that relate to mechanistic aspects I used Time Resolved X-ray Absorption Spectroscopy, Small Angle X-ray Scattering, and Atomic Force Microscopy. These biophysical tools were combined with biochemistry, hydrodynamic characterization (SEC, AUC, DLS), spectroscopy (CD, Fluorescence, Raman) and computational modeling.

Published by Gabriel Rosenblum

Molecular structures and dynamics of the stepwise activation mechanism of a matrix metalloproteinase zymogen: challenging the cysteine switch dogma.

Rosenblum G, Meroueh S, Toth M, Fisher JF, Fridman R, Mobashery S, and Sagi I

Journal of the American Chemical Society 129(44):13566, 2007 Nov 7 - Who cited this? | PubMed ID: 17929919 | Fulltext

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Insights into the structure and domain flexibility of full-length pro-matrix metalloproteinase-9/gelatinase B.

Rosenblum G, Van den Steen PE, Cohen SR, Grossmann JG, Frenkel J, Sertchook R, Slack N, Strange RW, Opdenakker G, and Sagi I

Structure (London, England : 1993) 15(10):1227, 2007 Oct - Who cited this? | PubMed ID: 17937912 | Fulltext

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Pronounced diversity in electronic and chemical properties between the catalytic zinc sites of tumor necrosis factor-alpha-converting enzyme and matrix metalloproteinases despite their high structural similarity.

Solomon A, Rosenblum G, Gonzales PE, Leonard JD, Mobashery S, Milla ME, and Sagi I

The Journal of biological chemistry 279(30):31646, 2004 Jul 23 - Who cited this? | PubMed ID: 15102849 | Fulltext

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Structural basis for potent slow binding inhibition of human matrix metalloproteinase-2 (MMP-2).

Rosenblum G, Meroueh SO, Kleifeld O, Brown S, Singson SP, Fridman R, Mobashery S, and Sagi I

The Journal of biological chemistry 278(29):27009, 2003 Jul 18 - Who cited this? | PubMed ID: 12679334 | Fulltext

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Ribosomal crystallography: from poorly diffracting microcrystals to high-resolution structures.

Gluehmann M, Zarivach R, Bashan A, Harms J, Schluenzen F, Bartels H, Agmon I, Rosenblum G, Pioletti M, Auerbach T, Avila H, Hansen HA, Franceschi F, and Yonath A

Methods (San Diego, Calif.) 25(3):292, 2001 Nov - Who cited this? | PubMed ID: 11860283 | Fulltext

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Education

School:
Tel-Hai college 1997-2000
BSc Cum Laude Biotechnology