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Papers authored by Stemmer WP

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Multivalent avimer proteins evolved by exon shuffling of a family of human receptor domains.

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Silverman J, Liu Q, Bakker A, To W, Duguay A, Alba BM, Smith R, Rivas A, Li P, Le H, Whitehorn E, Moore KW, Swimmer C, Perlroth V, Vogt M, Kolkman J,

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Nature biotechnology 23(12):1556-61, 2005 Dec - Who cited this? | PubMed ID: 16299519 | Fulltext

Diverse plasmid DNA vectors by directed molecular evolution of cytomegalovirus promoters.

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Wright A, Semyonov A, Dawes G, Crameri A, Lyons R, Stemmer WP, Apt D, and Punnonen J

Human gene therapy 16(7):881-92, 2005 Jul - Who cited this? | PubMed ID: 16000069 | Fulltext

Optimized expression and specific activity of IL-12 by directed molecular evolution.

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Leong SR, Chang JC, Ong R, Dawes G, Stemmer WP, and Punnonen J

Proceedings of the National Academy of Sciences of the United States of America 100(3):1163-8, 2003 Feb 4 - Who cited this? | PubMed ID: 12529500 | Fulltext

Genome shuffling of Lactobacillus for improved acid tolerance.

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Patnaik R, Louie S, Gavrilovic V, Perry K, Stemmer WP, Ryan CM, and del Cardayré S

Nature biotechnology 20(7):707-12, 2002 Jul - Who cited this? | PubMed ID: 12089556 | Fulltext

Evolution of a human immunodeficiency virus type 1 variant with enhanced replication in pig-tailed macaque cells by DNA shuffling.

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Pekrun K, Shibata R, Igarashi T, Reed M, Sheppard L, Patten PA, Stemmer WP, Martin MA, and Soong NW

Journal of virology 76(6):2924-35, 2002 Mar - Who cited this? | PubMed ID: 11861859 | Fulltext

Nontransgenic crops from transgenic plants.

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Keenan RJ and Stemmer WP

Nature biotechnology 20(3):215-6, 2002 Mar - Who cited this? | PubMed ID: 11875408 | Fulltext

How to publish DNA sequences with copyright protection.

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Stemmer WP

Nature biotechnology 20(3):217, 2002 Mar - Who cited this? | PubMed ID: 11875409 | Fulltext

Genome shuffling leads to rapid phenotypic improvement in bacteria.

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Zhang YX, Perry K, Vinci VA, Powell K, Stemmer WP, and del Cardayré SB

Nature 415(6872):644-6, 2002 Feb 7 - Who cited this? | PubMed ID: 11832946 | Fulltext

Novel enzyme activities and functional plasticity revealed by recombining highly homologous enzymes.

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Raillard S, Krebber A, Chen Y, Ness JE, Bermudez E, Trinidad R, Fullem R, Davis C, Welch M, Seffernick J, Wackett LP, Stemmer WP, and Minshull J

Chemistry & biology 8(9):891-8, 2001 Sep - Who cited this? | PubMed ID: 11564557 | Fulltext

Directed evolution of proteins by exon shuffling.

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Kolkman JA and Stemmer WP

Nature biotechnology 19(5):423-8, 2001 May - Who cited this? | PubMed ID: 11329010 | Fulltext

Predicting the emergence of antibiotic resistance by directed evolution and structural analysis.

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Orencia MC, Yoon JS, Ness JE, Stemmer WP, and Stevens RC

Nature structural biology 8(3):238, 2001 Mar - Who cited this? | PubMed ID: 11224569 | Fulltext

Breeding of retroviruses by DNA shuffling for improved stability and processing yields.

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Powell SK, Kaloss MA, Pinkstaff A, McKee R, Burimski I, Pensiero M, Otto E, Stemmer WP, and Soong NW

Nature biotechnology 18(12):1279-82, 2000 Dec - Who cited this? | PubMed ID: 11101807 | Fulltext

Molecular breeding of viruses.

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Soong NW, Nomura L, Pekrun K, Reed M, Sheppard L, Dawes G, and Stemmer WP

Nature genetics 25(4):436-9, 2000 Aug - Who cited this? | PubMed ID: 10932190 | Fulltext

Improving the catalytic activity of a thermophilic enzyme at low temperatures.

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Merz A, Yee MC, Szadkowski H, Pappenberger G, Crameri A, Stemmer WP, Yanofsky C, and Kirschner K

Biochemistry 39(5):880-9, 2000 Feb 8 - Who cited this? | PubMed ID: 10653631 | Fulltext

Molecular breeding: the natural approach to protein design.

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Ness JE, Del Cardayré SB, Minshull J, and Stemmer WP

Advances in protein chemistry 55:261-92, 2000 - Who cited this? | PubMed ID: 11050936 | Fulltext

DNA shuffling of subgenomic sequences of subtilisin.

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Ness JE, Welch M, Giver L, Bueno M, Cherry JR, Borchert TV, Stemmer WP, and Minshull J

Nature biotechnology 17(9):893-6, 1999 Sep - Who cited this? | PubMed ID: 10471932 | Fulltext

Evolution of a cytokine using DNA family shuffling.

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Chang CC, Chen TT, Cox BW, Dawes GN, Stemmer WP, Punnonen J, and Patten PA

Nature biotechnology 17(8):793-7, 1999 Aug - Who cited this? | PubMed ID: 10429246 | Fulltext

Protein evolution by molecular breeding.

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Minshull J and Stemmer WP

Current opinion in chemical biology 3(3):284-90, 1999 Jun - Who cited this? | PubMed ID: 10359711 | Fulltext

Colorless green ideas...

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Tobin M, Affholter JA, Stemmer WP, and Minshull J

Nature biotechnology 17(4):333-4, 1999 Apr - Who cited this? | PubMed ID: 10207877 | Fulltext

Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling.

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Christians FC, Scapozza L, Crameri A, Folkers G, and Stemmer WP

Nature biotechnology 17(3):259-64, 1999 Mar - Who cited this? | PubMed ID: 10096293 | Fulltext

DNA shuffling of a family of genes from diverse species accelerates directed evolution.

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Crameri A, Raillard SA, Bermudez E, and Stemmer WP

Nature 391(6664):288-91, 1998 Jan 15 - Who cited this? | PubMed ID: 9440693 | Fulltext

Applications of DNA shuffling to pharmaceuticals and vaccines.

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Patten PA, Howard RJ, and Stemmer WP

Current opinion in biotechnology 8(6):724-33, 1997 Dec - Who cited this? | PubMed ID: 9425664 | Fulltext

Molecular evolution of an arsenate detoxification pathway by DNA shuffling.

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Crameri A, Dawes G, Rodriguez E, Silver S, and Stemmer WP

Nature biotechnology 15(5):436-8, 1997 May - Who cited this? | PubMed ID: 9131621 | Fulltext

Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening.

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Zhang JH, Dawes G, and Stemmer WP

Proceedings of the National Academy of Sciences of the United States of America 94(9):4504-9, 1997 Apr 29 - Who cited this? | PubMed ID: 9114019 | Fulltext

Improved green fluorescent protein by molecular evolution using DNA shuffling.

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Crameri A, Whitehorn EA, Tate E, and Stemmer WP

Nature biotechnology 14(3):315-9, 1996 Mar - Who cited this? | PubMed ID: 9630892 | Fulltext

Affinity selective isolation of ligands from peptide libraries through display on a lac repressor "headpiece dimer".

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Gates CM, Stemmer WP, Kaptein R, and Schatz PJ

Journal of molecular biology 255(3):373-86, 1996 Jan 26 - Who cited this? | PubMed ID: 8568883 | Fulltext

Construction and evolution of antibody-phage libraries by DNA shuffling.

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Crameri A, Cwirla S, and Stemmer WP

Nature medicine 2(1):100-2, 1996 Jan - Who cited this? | PubMed ID: 8564822 | Fulltext

The evolution of molecular computation.

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Stemmer WP

Science (New York, N.Y.) 270(5241):1510, 1995 Dec 1 - Who cited this? | PubMed ID: 7491501 | Fulltext

Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides.

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Stemmer WP, Crameri A, Ha KD, Brennan TM, and Heyneker HL

Gene 164(1):49-53, 1995 Oct 16 - Who cited this? | PubMed ID: 7590320 | Fulltext

Combinatorial multiple cassette mutagenesis creates all the permutations of mutant and wild-type sequences.

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Crameri A and Stemmer WP

BioTechniques 18(2):194-6, 1995 Feb - Who cited this? | PubMed ID: 7727111 | Fulltext

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution.

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Stemmer WP

Proceedings of the National Academy of Sciences of the United States of America 91(22):10747-51, 1994 Oct 25 - Who cited this? | PubMed ID: 7938023 | Fulltext

Rapid evolution of a protein in vitro by DNA shuffling.

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Stemmer WP

Nature 370(6488):389-91, 1994 Aug 4 - Who cited this? | PubMed ID: 8047147 | Fulltext

10(20)-fold aptamer library amplification without gel purification.

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Crameri A and Stemmer WP

Nucleic acids research 21(18):4410, 1993 Sep 11 - Who cited this? | PubMed ID: 7692403 | Fulltext

Selection of an active single chain Fv antibody from a protein linker library prepared by enzymatic inverse PCR.

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Stemmer WP, Morris SK, and Wilson BS

BioTechniques 14(2):256-65, 1993 Feb - Who cited this? | PubMed ID: 8431292 | Fulltext

Increased antibody expression from Escherichia coli through wobble-base library mutagenesis by enzymatic inverse PCR.

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Stemmer WP, Morris SK, Kautzer CR, and Wilson BS

Gene 123(1):1-7, 1993 Jan 15 - Who cited this? | PubMed ID: 8422991 | Fulltext

Enzymatic inverse PCR: a restriction site independent, single-fragment method for high-efficiency, site-directed mutagenesis.

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Stemmer WP and Morris SK

BioTechniques 13(2):214-20, 1992 Aug - Who cited this? | PubMed ID: 1327007 | Fulltext

A 20-minute ethidium bromide/high-salt extraction protocol for plasmid DNA.

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Stemmer WP

BioTechniques 10(6):726, 1991 Jun - Who cited this? | PubMed ID: 1652261 | Fulltext

Reassembly of a fimbrial hemagglutinin from Pseudomonas solanacearum after purification of the subunit by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

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Young DH, Stemmer WP, and Sequeira L

Applied and environmental microbiology 50(3):605-10, 1985 Sep - Who cited this? | PubMed ID: 2866754 | Fulltext

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