AIM OF THE STUDY:
Traditional Chinese herb Angong Niuhuang
Pill (AGNHP) is a famous preparation for
neurological diseases; Qingkailing injection (QKL), an extract of AGNHP has similar clinical applications. This investigation was designed to further elucidate the neuroprotective effect of QKL on
intracerebral hemorrhage (ICH). MATERIALS AND METHODS: ICH was produced in adult
Sprague-Dawley rats by injection of
collagenase IV. Three incremental doses of QKL injection including low-(0.5 ml/kg), moderate-(1 ml/kg) and high-dosage (2 ml/kg) were administered twice, 3 and 12h following ICH.
TUNEL and caspase-3 activity were measured at 1d after ICH, and apomorphine-induced rotation was evaluated at 1d, 7d, 14 d and 28 d. RESULTS: Administration of high-dose QKL inhibited
TUNEL positive cells (p<0.05) and caspase-3 activity (p<0.05) at 1d following ICH, and reduced apomorphine-induced rotation at 1d (p<0.01), 7d, 14 d and 28 d (p<0.05), compared with the controls. However, QKL in a low or moderate dose had no such effect. CONCLUSION: QKL reduced
brain damage of
intracerebral hemorrhage through inhibiting
apoptosis, which suggested a potential intervention for ICH patients.
