AIMS/HYPOTHESIS: Additional susceptibility loci for
type 2 diabetes have been identified by a
meta-analysis of
genome-wide association studies (GWASs) in European populations. To examine further the roles of these new loci, we performed a replication study for the association of these
single-nucleotide polymorphism (
SNP) loci with the disease in three independent Japanese populations. METHODS: We genotyped seven of the 11
SNPs that emerged in stage 2 of the
meta-analysis for European GWASs (rs864745 in
JAZF1, rs12779790 near
CDC123/CAMK1D, rs7961581 near
TSPAN8/
LGR5, rs4607103 near
ADAMTS9, rs10923931 in
NOTCH2, rs1153188 near DCD and rs9472138 near
VEGFA) for three independent Japanese populations (first set, 1,630
type 2 diabetes patients vs 1,064 controls; second set, 1,272
type 2 diabetes patients vs 856 controls; third set, 486
type 2 diabetes patients vs 936 controls) using a TaqMan
assay. The association of the
SNP loci in each population was analysed using a
logistic regression analysis, adjusting for age, sex and BMI, and the data were evaluated by a
meta-analysis. RESULTS: A
meta-analysis for the three
case-control studies identified a nominal association of rs864745 in
JAZF1 with
type 2 diabetes (OR 1.148, 95% CI 1.034-1.275, p = 0.0098, corrected p = 0.069). The association of other loci did not reach
statistically significant levels (nominal p > 0.05). CONCLUSIONS/INTERPRETATION: From these results the contribution of these seven loci in conferring susceptibility to
type 2 diabetes is considered minor in the Japanese population, if they are present.
